A Gill Filament EROD Assay
نویسنده
چکیده
منابع مشابه
Gill EROD Activity in Fish A Biomarker for Waterborne Ah-receptor Agonists
List of papers This thesis is based on the following papers, which will be referred to in the text by their Roman numerals.rigtsen K, Jørgensen EH. 2003. EROD activity in gills of anadromous and marine fish as a biomarker of dioxin-like pollutants.rome P4501A induction in rainbow trout gills and liver following exposure to waterborne indigo, benzo(a)pyrene and 3,3',4,4',5-pentachlorobiphenyl. i...
متن کاملCell-specific CYP1A expression and benzo[a]pyrene adduct formation in gills of rainbow trout (Oncorhynchus mykiss) following CYP1A induction in the laboratory and in the field.
The effect of cytochrome P4501A (CYP1A) induction on cell-specific benzo[a]pyrene (BaP) adduct formation was studied in rainbow trout (Oncorhynchus mykiss) gills. Fish preexposed to beta-naphthoflavone (betaNF) or caged in a polluted river were exposed to waterborne 3H-benzo[a]pyrene (3H-BaP). The 3H-benzo[a]pyrene adducts in the gill filaments were localized by autoradiography and CYP1A protei...
متن کاملCultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants.
A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gil...
متن کاملMorphological variation and Taxonomic validation of gill raker characteristics in Caspian marine shad (Clupeidae: Alosa braschnikowi)
We used 50 specimens of Alosa braschnikowi (A. brschnikowi) to study the growth effect on Gill raker morphology. Gill rakers counted as 26-47 which increased in relation to increase in TL (P<0.05). Gill raker lengths and the length:width ratio decreased with a high coefficient of relationships (R2=0.818 and 0.634 subsequently) regarding the increase of TL. The proportion of gill raker length to...
متن کاملOptimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces)
Cytochrome P450 monooxygenase 1A (CYP1A) is induced by several planar toxic compounds, for example, polychlorinated biphenyls (PCBs) and the induction of this protein is often measured in terms of CYP1A-mediated 7-ethoxyresorufin-O-deethylase (EROD) activity. This study was aimed at developing this assay in the Cape hake species Merluccius capensis and Merluccius paradoxus (considered one stock...
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